First detection and origin of multi-drug resistant Klebsiella pneumoniae ST15 harboring OXA-48 in South America

First detection and origin of multi-drug resistant Klebsiella pneumoniae ST15 harboring OXA-48 in South America

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ABSTRACT: Objectives: The emergence and spread of carbapenem resistant clones is of major concern for global health.This study aimed to characterize the first detected Klebsiella pneumoniae ST15 harboring the epidemic carbapenemase OXA-48 in South America.Methods: During a routine colonization screening with carbapenem-resistant bacteria, one K.pneumoniae strain (CGHM01) was isolated from the urine of a hospitalized patient suffering from a neurodegenerative disease in Uruguay.

We used long-read whole-genome sequencing and a phylogenomic approach to characterize the emergence of K.pneumoniae CGHM01.Results: K.pneumoniae CGHM01 is a camo iphone se case multi-drug resistant strain carrying an IncL/M plasmid that encodes the carbapenemase gene blaOXA-48 within the Tn1999.

2 transposon.Also, it carries an IncR plasmid harboring a class I integron with an array fleshlight automatique of antibiotic resistance genes including the extended-spectrum beta-lactamase blaCTX-M-15.Two copies of blaCTX-M-15 were also inserted in different positions of the chromosome.CGHM01 belongs to a ST15 sublineage that likely originated in continental Spain around 2012.

Conclusions: The asymptomatic carriage of this strain in the urinary tract warns of difficulties for detection and reporting of emerging carbapenem-resistant clones in new geographic areas where these are not endemic.